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spectral flow cytometry  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences spectral flow cytometry
    Spectral Flow Cytometry, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 4460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aurora+spectral+flow+cytometry/Aurora/pm41896813-173-9-12
    Average 99 stars, based on 4460 article reviews
    spectral flow cytometry - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry. .. Data were exported and analyzed using FlowJo software (v10.9).

    Article Title: Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis.
    Article Snippet: .. Data was recorded using a Cytek Aurora Spectral Flow Cytometry (Cytek). .. Detailed antibodies information and dillution as followed: CD3 (1:200, BioLegend, Cat# 317340), anti-CD11c (1:200, BioLegend, Cat# 337220), anti-CD14 (1:200, BioLegend, Cat# 301822), antiCD19 (1:200, BioLegend, Cat# 302226), anti-CD20 (1:200, BioLegend, Cat# 302322), anti-CD56 (1:200, BioLegend, Cat# 318316), and anti-CD206 (1:200, BioLegend, Cat# 321132).

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury
    Article Snippet: .. All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software. ..

    Article Title: Coated oncolytic viruses based "double strike" strategy triggering CD19 CAR-T therapy in gastrointestinal tumors.
    Article Snippet: The efficacy of chimeric antigen receptor T (CAR-T) cell therapy in solid tumors is limited by the scarcity of stable antigen targets.. To enhance CAR-T cell immunotherapy for solid tumors, we developed an integrated system that simultaneously introduces a surface antigen target for CAR-T cells and remodels the immunosuppressive tumor immune microenvironment (TIME) using an oncolytic adenovirus (OVs).. This system leverages B cell membrane-derived CD19 as an artificial antigen target on tumor cells and enables in situ production of an αCD3e-αEpCAM bispecific T cell engager (BiTEs), which further strengthens the binding and cytotoxicity of CD19 CAR-T cells to cancer cells.

    Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic.
    Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Aurora Spectral Flow Cytometry (Cytek Biosciences). .. FloJo v10.10 software (BD) was used for analysis.

    Article Title: Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis
    Article Snippet: .. Data was recorded using a Cytek Aurora Spectral Flow Cytometry (Cytek). .. Bone marrow cells were harvested from the femurs and tibias of 8-week-old female C57/B6L mice and centrifuged at 300 × g for 5 min to obtain the cell pellet, followed by red cells depletion with ACK lysing buffer (Thermo Fisher, Cat# A1049201) and incubated at room temperature in the dark for 10 min.

    Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic
    Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Aurora Spectral Flow Cytometry (Cytek Biosciences). .. FloJo v10.10 software (BD) was used for analysis.

    Article Title: Probiotic-mediated tumor microenvironment reprogramming with protease-sensitive interleukin-15 and photothermal therapy.
    Article Snippet: .. Finally, the cells were detected using Cytek Aurora spectral flow cytometry and analyzed by Flowjo software (TreeStar, 10.6.2). ..

    Staining:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry. .. Data were exported and analyzed using FlowJo software (v10.9).

    Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic.
    Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Aurora Spectral Flow Cytometry (Cytek Biosciences). .. FloJo v10.10 software (BD) was used for analysis.

    Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic
    Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Aurora Spectral Flow Cytometry (Cytek Biosciences). .. FloJo v10.10 software (BD) was used for analysis.

    Incubation:

    Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F
    Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Aurora Spectral Flow Cytometry. .. Data were exported and analyzed using FlowJo software (v10.9).

    Software:

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury
    Article Snippet: .. All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software. ..



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    (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow <t>cytometry</t> plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.
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    Cytek Biosciences cytektm aurora spectral flow cytometry
    (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow <t>cytometry</t> plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.
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    Image Search Results


    (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow cytometry plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.

    Journal: bioRxiv

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury

    doi: 10.64898/2026.03.23.713833

    Figure Lengend Snippet: (A) Experimental design. (B-D) Relative fold-change hippocampal gene expression of Cxcl1, Ccl2, Il1b . (E) Representative flow cytometry plots for Ly6C + monocytes, Ly6G + neutrophils and CD11c + dendritic cells. (F) Back-gating strategy to identify IL-1β-producing innate immune cells. (G-K) Bar graphs illustrate the absolute numbers of Ly6G + neutrophils (G), Ly6C + monocytes (H), CD11c + dendritic cells (I), IL-1β-producing neutrophils (J), IL-1β-producing monocytes (K). Data = Mean ± SEM (n=3-4 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 3hpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 6hpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 12hpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Abbreviations: Hp, hippocampus.

    Article Snippet: All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software.

    Techniques: Gene Expression, Flow Cytometry

    (A) Experimental design. (B-D) Relative fold-change expression levels of Cxcl10, Icam1, Itgb2 . (E) Flow cytometry plots of CD4 + , CD8 + , γδ + , and NK + T cells. (F) Representative flow cytometry plots for IL-17 and IFN-γ production by different T cell subsets. (G-O) Bar graphs illustrate the absolute numbers of CD4+ T cells (G), CD8+ T cells (H), NK + T cells (I), γδ + T cells (J), IFN-γ-producing CD4 + T cells (K), IFN-γ-producing CD8 + T cells (L), IFN-γ-producing NK + T cells (M), IL-17-producing γδ + T cells (N), and IFN-γ-producing γδ + T cells (O). Data = Mean ± SEM (n=6 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 1dpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 3dpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 10dpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons. Abbreviations: Hp, hippocampus.

    Journal: bioRxiv

    Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury

    doi: 10.64898/2026.03.23.713833

    Figure Lengend Snippet: (A) Experimental design. (B-D) Relative fold-change expression levels of Cxcl10, Icam1, Itgb2 . (E) Flow cytometry plots of CD4 + , CD8 + , γδ + , and NK + T cells. (F) Representative flow cytometry plots for IL-17 and IFN-γ production by different T cell subsets. (G-O) Bar graphs illustrate the absolute numbers of CD4+ T cells (G), CD8+ T cells (H), NK + T cells (I), γδ + T cells (J), IFN-γ-producing CD4 + T cells (K), IFN-γ-producing CD8 + T cells (L), IFN-γ-producing NK + T cells (M), IL-17-producing γδ + T cells (N), and IFN-γ-producing γδ + T cells (O). Data = Mean ± SEM (n=6 per time point); differences vs. Sham *p<0.05, **p<0.01, ***p<0.001; differences vs. 1dpi $ p<0.05, $$ p<0.01, $$$ p<0.001; differences vs. 3dpi # p<0.05, ## p<0.01, ### p<0.001; differences vs. 10dpi § p<0.05, §§ p<0.01, §§§ p<0.001 by one-way ANOVA with Tukey’s multiple comparisons. Abbreviations: Hp, hippocampus.

    Article Snippet: All flow cytometric data were acquired using Cytek Aurora Spectral flow cytometry with SpectroFlo software.

    Techniques: Expressing, Flow Cytometry