spectral flow cytometry (Cytek Biosciences)
Structured Review
Spectral Flow Cytometry, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 4460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aurora+spectral+flow+cytometry/Aurora/pm41896813-173-9-12
Average 99 stars, based on 4460 article reviews
Images
Related Articles
Flow Cytometry:Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Article Title: Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis. Article Snippet: .. Data was recorded using a Cytek Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury Article Snippet: .. All flow cytometric data were acquired using Cytek Article Title: Coated oncolytic viruses based "double strike" strategy triggering CD19 CAR-T therapy in gastrointestinal tumors. Article Snippet: The efficacy of chimeric antigen receptor T (CAR-T) cell therapy in solid tumors is limited by the scarcity of stable antigen targets.. To enhance CAR-T cell immunotherapy for solid tumors, we developed an integrated system that simultaneously introduces a surface antigen target for CAR-T cells and remodels the immunosuppressive tumor immune microenvironment (TIME) using an oncolytic adenovirus (OVs).. This system leverages B cell membrane-derived CD19 as an artificial antigen target on tumor cells and enables in situ production of an αCD3e-αEpCAM bispecific T cell engager (BiTEs), which further strengthens the binding and cytotoxicity of CD19 CAR-T cells to cancer cells. Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic. Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Article Title: Airway immune profiles and therapeutic implications of IGF1 in eosinophilic granulomatosis with polyangiitis Article Snippet: .. Data was recorded using a Cytek Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Article Title: Probiotic-mediated tumor microenvironment reprogramming with protease-sensitive interleukin-15 and photothermal therapy. Article Snippet: .. Finally, the cells were detected using Cytek Staining:Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic. Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Article Title: Host-directed broad-spectrum immunotherapeutic strategy for respiratory infections: Heat-killed Caulobacter crescentus (HKCC) as an innate-immune based biotherapeutic/postbiotic Article Snippet: .. The samples were analyzed on the same day as staining using Cytek Incubation:Article Title: Apolipoprotein E drives microglia activation in the development of autoimmune uveitis through up-regulation of peptidyl prolyl isomerase F Article Snippet: For surface receptor staining, the prepared cell suspension was treated with a mouse Fc receptor blocking agent (Miltenyi Biotec, 130-092-575, diluted 1:100) and incubated at 4°C for 20 min, followed by staining with specific antibodies at 4°C in the dark for 20 min. .. Cells were then fixed and permeabilized using a flow cytometry fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5223-56 and 00-5123-43) at room temperature in the dark for 10 min. For intracellular cytokine staining, the cells were subsequently incubated with a flow cytometry permeabilization buffer (Thermo Fisher Scientific, 00-8333-56), followed by the addition of specific antibodies and incubation at room temperature in the dark for 20 min. Acquisition and compensation were performed by Cytek Software:Article Title: Impact of innate immune activation on T cell dynamics and functional recovery following traumatic brain injury Article Snippet: .. All flow cytometric data were acquired using Cytek |
